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Microarray Analysis is a natural outgrowth of the author's teaching and research into the fi eld of microarray processing, a fast growing and developing fi eld. There has been a reduction in the genominomics of sequencing of DNA and proteins: from $3 billion in HGP costs down to $1000 per genome in 2014 using next-generation sequencers. Different sequencing methods in place and various applications of these methods are illustrated. Commercially available Next-Generation Sequencers are compared side-side by side. This book will show how the biochemical reactions involved in the different steps of the cure process can be studied using microarray methods. Examples will be provided throughout the text of various disease states, including the spread of cystic fi brosis in children, consanguineous marriages in Saudi Arabia, genetic disorders, and autoimmune disorders. The protocols from sample preparation to confocal scanning microscopy and detectors using photo multiplier tubes to statistical data analysis methods will be discussed in detail. The Polymerase chain reaction, a technique used to amplify and simultaneously quantify a targeted DNA molecule is explored in relation to the best surface to be used for Microarray Analysis. Sanger sequencing, sequencing by synthesis, sequencing by ligation, single molecule sensing, DNA passage through nanopores in graphene are some methods used for obtaining sequence distribution information.
Publisher's Note: Products purchased from Third Party sellers are not guaranteed by the publisher for quality, authenticity, or access to any online entitlements included with the product. State-of-the-art nanostructuring principles, methods, and aplications Synthesize, characterize, and deploy highly miniaturized components using the theories and techniques contained in this comprehensive resource. Written by a nanotechnology expert, this authoritative volume covers the latest advances along with detailed schematics and real-world applications in engineering and the life sciences. Inside, 37 different nanostructuring methods and 16 different kinds of nanostructures are discussed. Nanostructuring Operations in Nanoscale Science and Engineering explains how to manufacture high-purity fullerenes, assemble carbon nanotubes, and use nanofluids and nanowires. You will also learn how to develop high-performance biochips, work with biomimetics, and design molecular machines. The book includes 540 end-of-chapter review questions to reinforce the material covered. Learn how to: Produce fullerenes using metallurgic, solar, and electric arc methods Use arc discharge, laser ablation, CVD, and HIPCO to create CNTs Build nanostructures with vacuum synthesis, gas evaporation, and lithography Work with quantum dots, polymer thin films, nanofluids, and nanoceramics Develop biochips, biological nanovalves, and molecular machines Mimic biological characteristics and organic self-repair using biomimetics Model nanoscale effects with relativistic and Laplace transforms Characterize nanoscale material using x-ray and helium ion microscope
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